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Objective To summarize the SPECT/CT manifestation of spondylitis caused by Brucells infection and to evaluate the diagnostic value.Methods From June 2012 to October 2015,a total of 28 patients (14 males,14 females,average age 46.4 years) with Brucellosis spondylitis confirmed by laboratory test and pathology were included.The images of whole-body bone scan and SPECT/CT fusion imaging were retrospectively analyzed.According to the pathological and serologic test results,the diagnostic efficacy of imaging was calculated.x2 test was used.Results Most of the Brucellosis spondylitis happened in the lumbar(76.7％,43/56),and the most common locations were L3,L4,L5 (72.1％,31/43).Two or more involved consecutive vertebra were found in 71.4％ (20/28) of the patients.Moderate radioactive distribution was showed in 89.2％ (50/56) of lesions,high radioactive distribution was showed in 5.4％ (3/56) of lesions,and mild radioactive distribution was showed in the rest 3 lesions.Thirty-three lesions(58.9％,33/56) had diffuse increased radioactivity uptake in the affected vertebra,and 32.1％(18/56) showed diffuse increased radioactivity at the superior and inferior margin of the vertebra;only 8.9％ (5/56) of lesions were on one side of the vertebral bodies.The SPECT/CT results were as follows:(1) Bone destruction was showed in 80.4％ (45/56) of lesions,and the edge of the lesion was clear.(2) For 66.7％ (30/45) of lesions,bone hyperplasia was seen along with bone destruction and moderate radioactivity concentration on the edge of destruction area.(3) The damage of the intervertebral disc was mild,and the vertebral abscess was relatively rare (5.4％,3/56).The diagnostic accuracy of SPECT/CT was statistically higher than that of whole-body bone scan:67.8％(38/56) vs 96.2％(54/56);x2=13.1,P＜0.05.Conclusion SPECT/CT imaging has a higher diagnostic efficiency than whole-body bone scan in Brucellosis spondylitis.
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In recent years,T helper cell 17(Th17),regulatory T cell(Treg) and podocyte injury attrac-ted widespread attention in the pathogenesis of primary glomerular disease. Th17 cells have the function of re-cruiting neutrophils and macrophages to the infected tissue through the secretion of cytokines such as IL-17. Treg cells have immune function, mediated immune tolerance, protecting the body against inflammatory injury. The imbalance of Th17 cells increase and Treg cells decrease could play an important role in the pathogenesis and progression of primary glomerular disease. As the important part of the glomerular filtration barrier,podocyte be-comes the focus in recent years. Study on relationship among Th17,Treg,podocyte injury and primary glomeru-lar disease will provide more theoretical basis for the prevention and treatment of primary glomerular disease.
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BACKGROUND:Different sources of stem cells have different molecular characteristics and growth characteristics;therefore, there are some differences in therapeutic mechanisms and effects. OBJECTIVE:To compare mesenchymal stem cells growth characteristics form two sources. METHODS:Mesenchymal stem cells from the umbilical cord and the embryonic liver were isolated and cultured. Passage 5 cells were used to observe the cellmorphology, calculate the doubling time of cellpopulation-doubling time, identify surface markers and determine the differentiation capacity. Mesenchymal stem cells from the umbilical cord were subcultured to passages 10 and 15, and cellcurves were drawn and population doubling time was calculated. RESULTS AND CONCLUSION:Mesenchymal stem cells from these two sources in logarithmic phase were fusiform and grew spiral y with osteogenic, adipogenic, and chondrogenic capacities. The growth curves of cells were both S-shaped. At passage 5, the doubling time was (34.37±0.31) hours for embryonic liver-derived mesenchymal stem cells and (35.63±0.38) hours for umbilical cord-derived mesenchymal stem cells, and there was no significant difference (P>0.05). However, the population doubling time of passages 10 and 15 umbilical cord-derived mesenchymal stem cells was (52.6±0.53) and (53.27±0.92) hours, respectively, which was significantly difference from that of passage 5 cells (P<0.05). The cellmorphology and growth curve from two sources are basical y the same. Embryonic liver-derived stem cells are smal er and proliferate faster than umbilical cord-derived mesenchymal stem cells, but no statistical difference is found between the two types.
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Objective To explore the relationship of human papilloma virus (HPV) genotype and loading dose with development of cervical intraepithelial neoplasia (CIN) or squamous cell carcinoma of the cervix ( SCC),and explore the relationship of HPV genotype and CIN or SCC.Methods One hundred and twenty four patients in Sun Yat-sen Memoral Hospital of Sun Yat-sen University with CIN or SCC from September 2005 to December 2010 were selected in this study.HPV DNA was detected by Hybrid capture Ⅱand flow-through hybridization and gene chip.The relationship between the infection of HPV and CIN or SCC was analyzed.The influencing factors of CIN or SCC were analyzed by logistic regression.Results The total detection rate of HPV was 75.8％,and it was 44.4％,70.0％,95.7％ and 76.2％ in CIN Ⅰ,CIN Ⅱ,CIN Ⅲ and SCC group,respectively.The detection rate of HPV in high-grade lesion group ( 84.5％ ) was higher than low-grade lesion group(44.4％ ).The median of HPV load decreased in order as CIN Ⅲ,SCC,CIN Ⅱ and CIN Ⅰgroup.Infection of multi-genotypes or single genotype of high-risk HPV accounted for 97.9％.In logistic regression,HPV loading dose had significant influence on degree of cervical lesion.Conclusions Infection of HPV is a main etiological factor for SCC.There is some kind of correlation between HPV loading dose and development of SCC.
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Objective To explore how to elevate the efficiency of fetal heart screening.Methods Volume data of 83 normal fetuses ranging between 20 to 24 gestational weeks were acquired by spatiotemporal image correlation (STIC) with the apical four-chamber view(4CV).Tomographic ultrasound imaging (TUI) was then used to present the different views of fetal hearts,and were compared with those aquired by 2-dimensional (2D) ultrasound.Results All the 83 volume datasets were obtained by STIC.(1) Qualification ratio of short axis view of the aorta (SAV) by TUI was higher than that of 2D (94.0％ vs 84.3％,x2 =5.57,P=0.042).While,in the other 8 planes,the qualification ration by STIC were higher than that of 2D,however no significant difference was found (P＞0.05).(2) In 4CV,5-chamber view (5CV),left ventricular outflow tract view (LVOT),right ventricular outflow tract view (RVOT) and SAV,STIC showed excellent consistency with 2D (Kappa =0.79,0.90,0.92,0.93 and 0.77,respectively).And in long axis view of the aortic arch (LAV-AoA),long axis view of the duct (LAV-DA),superior and inferior vena cava (SVC+IVC) and short axis view of the ventricle (SVV),STIC showed good agreement with 2D (Kappa=0.72,0.67,0.74 and 0.70,respectively).(3) Image collecting time of T2D,TSTIC,TTUI and TSTIC+TUI were (5.80±1.58) min,(0.85±0.18) min,(2.57±1.32) min and (3.29±1.13) min,respectively.There were significant difference between TSTIC and T2D(t=3.500,P=0.000) and between TSTIC+TUI and TTUI (t=2.877,P=0.001).Conclusion The technique of STIC-TUI used in this study can display the standard planes of fetal heart conveniently,clearly and in shorter time.It is helpful for improving the quality and efficiency of fetal heart screening.
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Objective To investigate clinical significance of counting follicles classification by three-dimensional imaging with sonography based automated volume calculation(SonoAVC)in the diagnosis of polycystic ovary syndrome(PCOS).Methods Eighty cases with PCOS were counted classified follicles and determined ovarian volume by three-dimensional(3D)imaging with SonoAVC method matched with 60 infertile women with fallopian tube or male factors as control.Main clinical.biological and other ultrasonographic markers were assessed during the early follicular phase,and the relationship between the follicle number range per ovary or the volume per ovary and the major hormonal features of PCOS was studied.Results Three-dimensional ultrasound imaging with SonoAVC methed provides a new path for objective quantitative assessment of follicle count.ovarian volume,total follicle numbers.The volume of (11 ±8)ml,total numbers of 27 ±14 follicle and number of22 ±19 follicle with diameter of≥2-＜6 mm in PCOS patients were significantly higher than(6 ±4)ml in ovarian volume.6 ±4 in total follicles and 2 ±3 in follicle with diameter of≥2-＜6 mm in controls(P＜0.05).while follicles were similar for the≥6-≤9mm range(P＞0.05).Total follicle numbers and follicles≥2-＜6 mm had significantly positive relationships with ovarian volume ( r= 0. 600, 0. 618, P＜0. 01 ) and level of testosterones ( r= 0. 364,0. 291, P＜0.05), follicles ≥2-＜6 mm also had significantly positive relationships with total follicle number (r=0. 916,P＜0. 01 ). The follicles within the ≥6 - ≤9 mm range was significantly and negatively related to ovarian volume and total follicle numbers ( r = - 0. 618, - 0. 263, all P = 0. 001 ), but no significantly related to the major hormonal features of PCOS. The ovarian volume was significantly positively related with luteinizing hormone ( LH)/follicle stimulating hormone (FSH) ratio ( r= 0. 282, P = 0. 010)but negatively related to FSH level (r = - 0. 226, P = 0. 042). Conclusions Ovarian volume, total follicle numbers and follicles ≥2 - ＜6 mm in PCOS patients were significantly higher than those in controls. The larger ovarian volume might produce more total follicle and follicles ≥ 2 - ＜6 mm. The higher level of testosterone might produce more total follicle probably, which mainly result in more follicles ≥2 -＜6 mm.These morphologically ultrasonographic characteristics could reflect pathophysiological changes in PCOS. Obviously, it has important clinical significance to count follicles in patients with PCOS by the threedimensional ultrasound imaging with SonoAVC method.
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Objective To study clinical features, diagnosis and treatment of allergic bronchopulmonary aspergillosis (ABPA) . Methods Clinical data and auxiliary examinations of eight patients with ABPA were analyzed, as well as relevant literatures were reviewed. Results All patients had symptom of cough, expectoration, out of breath and wheezing tale, seven of them with history of asthma, and all of them had increased peripheral blood eosinophil (EOS) count, serum levels of total immunoglobulin E (IgE) and lgE specific for Aspergillus fumigatus and showed positive prick test specific for Aspergillas fumigatus. Six of them showed infiltration shadows and six of them showed bronchiectasis in their chest CT images. Lung function test indicated obstructive ventilation dysfunction or mixed ventilation dysfunction with reversible airflow obstruction after administration of bronchodilator. Conclusions Diagnosis for ABPA should be made by integration of clinical features, increased blood eosinophil count and serum level of IgE, and result of prick test specific for Aspergillus fumigatus. Corticosteroid is a mainstay therapy for ABPA, ancillary with anti-fungi therapy. Patients with ABPA should be followed-up to prevent its recurrence.
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OBJECTIVE To study the distribution and drug resistance of the cancer patient′s pathogenic bacteria.METHODS The clinical nosocomial infection of 1451 cancer patients was analyzed by using the soft WHONET-5.RESULTS Of 955 strains isolated from sputa,the G-bacilli were 31.2%,and their main bacteria were Pseudomonas aeruginosa and Klebsiell pneumoniae,the G+ coccis were 31.2%,and their main bacteria were Staphylococcus epidermidis,and the fungi were 43.3%,and their main molds were Candida.albicans.Of 284 strains isolated form stool,the G-bacilli were 64.4%,and their main bacteria were Eschericha coli,the G+ cocci were 10.2% and their main bacteria were S.epidermidis,and fungi were 25.5%.Of 72 strains isolated from blood,the G-bacilli were 62.5%,and their main bacteria were E.coli,the G+ cocci were 30.5% and the fungi were 7.0%.Of 140 strains isolated from pharyngeal swab,the G-bacilli were 15.0%,and the G+ cocci were 43.0% and the fungi were 42.0%.The results of sensitivity tests showed as followed: The G-bacilli had a highly sensitive to imipenem,and had a high drug resistance against the first and second generation cephalosporin,ampicillin,piperacillin.The G+ cocci were highly sensitive to vancomycin and had a high drug resistance against oxacillin,penicillin,and erythromycio,the fungi had an obvious drug resistance against azoles.CONCLUSIONS It is high prevalence of ESBLs among MRS,and Staphylococcus.The application and selection of antibiotics must be based on the results of sensitivity tests,and the drug resistance of pathogenic bacteria must be controlled.